An organ-derived extracellular matrix triggers in situ kidney regeneration in a preclinical model

Kazuki Tajima, Hiroshi Yagi, Toshinori Morisaku, Kotaro Nishi, Hiroko Kushige, Hideaki Kojima, Hisanobu Higashi, Kohei Kuroda, Minoru Kitago, Shungo Adachi, Tohru Natsume, Kumiko Nishimura, Mototsugu Oya, Yuko Kitagawa

研究成果: Article査読

抄録

It has not been considered that nephrons regenerate in adult mammals. We present that an organ-derived extracellular matrix in situ induces nephron regeneration in a preclinical model. A porcine kidney-derived extracellular matrix was sutured onto the surface of partial nephrectomy (PN)-treated kidney. Twenty-eight days after implantation, glomeruli, vessels, and renal tubules, characteristic of nephrons, were histologically observed within the matrix. No fibrillogenesis was observed in the matrix nor the matrix-sutured kidney, although this occurred in a PN kidney without the matrix, indicating the structures were newly induced by the matrix. The expression of renal progenitor markers, including Sall1, Six2, and WT-1, within the matrix supported the induction of nephron regeneration by the matrix. Furthermore, active blood flow was observed inside the matrix using computed tomography. The matrix provides structural and functional foundations for the development of cell-free scaffolds with a remarkably low risk of immune rejection and cancerization.

本文言語English
論文番号18
ジャーナルnpj Regenerative Medicine
7
1
DOI
出版ステータスPublished - 2022 12月

ASJC Scopus subject areas

  • 医学(その他)
  • 生体医工学
  • 発生生物学
  • 細胞生物学

フィンガープリント

「An organ-derived extracellular matrix triggers in situ kidney regeneration in a preclinical model」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル