TY - JOUR
T1 - An RND-type multidrug efflux pump SdeXY from Serratia marcescens
AU - Chen, Jing
AU - Kuroda, Teruo
AU - Huda, Md Nazmul
AU - Mizushima, Tohru
AU - Tsuchiya, Tomofusa
N1 - Funding Information:
We thank Dr Manuel Varela of Eastern New Mexico University for critical reading of the manuscript prior to submission. This research was supported by a grant from the Ministry of Education, Science, Sport and Culture of Japan.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Objectives: Serratia marcescens, an important cause of nosocomial infections, shows intrinsic resistance to a wide variety of antimicrobial agents (multidrug resistance). Multidrug efflux pumps are often involved in the multidrug resistance in many bacteria. A study was undertaken to characterize the multidrug efflux pumps in S. marcescens. Methods: The genes responsible for the multidrug resistance phenotype in S. marcescens were cloned into Escherichia coli KAM32, a drug-hypersusceptible strain, for further analysis. Results: We cloned sdeXY genes and determined the nucleotide sequence. Clones that carried the sdeXY genes displayed reduced susceptibility to several antimicrobial agents including erythromycin, tetracycline, norfloxacin, benzalkonium chloride, ethidium bromide, acriflavine and rhodamine 6G. A protein similarity search using GenBank revealed that SdeY is a member of the resistance nodulation cell-division (RND) family of multidrug efflux proteins and SdeX is a member of the membrane fusion proteins. Introduction of sdeXY into E. coli cells possessing tolC, but not in cells lacking tolC, resulted in multidrug resistance. We observed energy-dependent ethidium efflux in cells of E. coliKAM32 possessing sdeXY and tolC. Conclusions: SdeXY is the first RND-type multidrug efflux pump to be characterized in multidrug-resistant S. marcescens.
AB - Objectives: Serratia marcescens, an important cause of nosocomial infections, shows intrinsic resistance to a wide variety of antimicrobial agents (multidrug resistance). Multidrug efflux pumps are often involved in the multidrug resistance in many bacteria. A study was undertaken to characterize the multidrug efflux pumps in S. marcescens. Methods: The genes responsible for the multidrug resistance phenotype in S. marcescens were cloned into Escherichia coli KAM32, a drug-hypersusceptible strain, for further analysis. Results: We cloned sdeXY genes and determined the nucleotide sequence. Clones that carried the sdeXY genes displayed reduced susceptibility to several antimicrobial agents including erythromycin, tetracycline, norfloxacin, benzalkonium chloride, ethidium bromide, acriflavine and rhodamine 6G. A protein similarity search using GenBank revealed that SdeY is a member of the resistance nodulation cell-division (RND) family of multidrug efflux proteins and SdeX is a member of the membrane fusion proteins. Introduction of sdeXY into E. coli cells possessing tolC, but not in cells lacking tolC, resulted in multidrug resistance. We observed energy-dependent ethidium efflux in cells of E. coliKAM32 possessing sdeXY and tolC. Conclusions: SdeXY is the first RND-type multidrug efflux pump to be characterized in multidrug-resistant S. marcescens.
KW - Multidrug efflux pump
KW - RND family
KW - S. marcescens
KW - SdeXY
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U2 - 10.1093/jac/dkg308
DO - 10.1093/jac/dkg308
M3 - Article
C2 - 12837741
AN - SCOPUS:0043029931
SN - 0305-7453
VL - 52
SP - 176
EP - 179
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 2
ER -
