An RND-type multidrug efflux pump SdeXY from Serratia marcescens

Jing Chen, Teruo Kuroda, Md Nazmul Huda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Objectives: Serratia marcescens, an important cause of nosocomial infections, shows intrinsic resistance to a wide variety of antimicrobial agents (multidrug resistance). Multidrug efflux pumps are often involved in the multidrug resistance in many bacteria. A study was undertaken to characterize the multidrug efflux pumps in S. marcescens. Methods: The genes responsible for the multidrug resistance phenotype in S. marcescens were cloned into Escherichia coli KAM32, a drug-hypersusceptible strain, for further analysis. Results: We cloned sdeXY genes and determined the nucleotide sequence. Clones that carried the sdeXY genes displayed reduced susceptibility to several antimicrobial agents including erythromycin, tetracycline, norfloxacin, benzalkonium chloride, ethidium bromide, acriflavine and rhodamine 6G. A protein similarity search using GenBank revealed that SdeY is a member of the resistance nodulation cell-division (RND) family of multidrug efflux proteins and SdeX is a member of the membrane fusion proteins. Introduction of sdeXY into E. coli cells possessing tolC, but not in cells lacking tolC, resulted in multidrug resistance. We observed energy-dependent ethidium efflux in cells of E. coliKAM32 possessing sdeXY and tolC. Conclusions: SdeXY is the first RND-type multidrug efflux pump to be characterized in multidrug-resistant S. marcescens.

本文言語English
ページ(範囲)176-179
ページ数4
ジャーナルJournal of Antimicrobial Chemotherapy
52
2
DOI
出版ステータスPublished - 2003 8月 1
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 微生物学(医療)
  • 感染症
  • 薬理学(医学)

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