An ultra-small fluid oscillation unit for pumping driven by self-organized three-dimensional bridging of pulsatile cardiomyocytes on elastic micro-piers

Nobuyuki Tanaka, Tadahiro Yamashita, Yaxiaer Yalikun, Satoshi Amaya, Asako Sato, Viola Vogel, Yo Tanaka

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Recent progress in microengineering has included the demonstration of various micropumps; however, these pumps are typically driven by an external energy sources such as electrical power source. Thus, there is a limitation to the integration of such pumps into microdevices. Here, we report fabrication of the world smallest autonomous hybrid pump powered by cardiomyocytes that self-organize into microtissues bridging PDMS made elastic microstructure. First, it was confirmed that cardiomyocytes formed several contractile bridges crossing the side walls of micro-groove and optimized the conditions under which they formed. Second, an actual ultra-small fluid oscilaltion unit for pumping (200 μm × 200 μm × 150 μm)was fabricated by embedding the micro-piers in a semi-closed microfabricated space filled with physiological buffer and closed with a cover glass lid on the device. Spontaneous and periodical oscillations of both micro-piers and the fluid in the device were confirmed. Simulation to understand the flow pattern and distribution of the flow velocity matches well with the experimental results. The theoretical flow rate assuming the use of ideal check valves was 1.0 nL/min. In the future, we expect this cardiomyocyte-driven device to be applied to applications such as in vivo micropumps, small-scale organs-on-a-chip and large-scale drug discovery assays.

本文言語English
ページ(範囲)256-264
ページ数9
ジャーナルSensors and Actuators, B: Chemical
293
DOI
出版ステータスPublished - 2019 8 15

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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