TY - JOUR
T1 - Analysis of cell accumulation mechanism in a rotational culture system
AU - Fukagata, Koji
AU - Furukawa, Katsuko S.
AU - Ushida, Takashi
N1 - Funding Information:
The authors are grateful to Drs. Nobuhide Kasagi, Yuji Suzuki, and Naoki Shika-zono (Department of Mechanical Engineering, The University of Tokyo) for fruitful discussion. This work was supported through the Grant-in-Aid for Scientific Research (S) by the Ministry of Education, Culture, Sports and Technology of Japan.
PY - 2011/4
Y1 - 2011/4
N2 - The accumulation mechanism of cells in a rotational culture device is investigated from the viewpoint of fluid mechanics. For simplicity, the deformation of the water surface is neglected and the cells are treated as spherical solid particles. From the numerical simulation of flow field with typical parameters used in the previous experiments, it is confirmed that the relative velocity of fluid induced by the rotational shaking is much smaller than the speed of rotation. From the analysis of particle equation of motion, it is found that the accumulation of cells toward the central region is found to be due to the interaction between the acceleration by rotational shaking and the drag force acting on the cells. The integral time scale for cell accumulation was estimated to be about 10 min for typical cases. The accumulation speed increases quadratically with the diameter of cell and the angular velocity of rotational shaking, which qualitatively support the previous experimental observation.
AB - The accumulation mechanism of cells in a rotational culture device is investigated from the viewpoint of fluid mechanics. For simplicity, the deformation of the water surface is neglected and the cells are treated as spherical solid particles. From the numerical simulation of flow field with typical parameters used in the previous experiments, it is confirmed that the relative velocity of fluid induced by the rotational shaking is much smaller than the speed of rotation. From the analysis of particle equation of motion, it is found that the accumulation of cells toward the central region is found to be due to the interaction between the acceleration by rotational shaking and the drag force acting on the cells. The integral time scale for cell accumulation was estimated to be about 10 min for typical cases. The accumulation speed increases quadratically with the diameter of cell and the angular velocity of rotational shaking, which qualitatively support the previous experimental observation.
KW - Tissue engineering
KW - cell culture
KW - fluid mechanics
KW - numerical simulation
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U2 - 10.1142/S0219519410003745
DO - 10.1142/S0219519410003745
M3 - Article
AN - SCOPUS:79960344730
SN - 0219-5194
VL - 11
SP - 407
EP - 421
JO - Journal of Mechanics in Medicine and Biology
JF - Journal of Mechanics in Medicine and Biology
IS - 2
ER -