Analysis of CSK homologous kinase (CHK/HYL) in hematopoiesis by utilizing gene knockout mice

Isao Hamaguchi, Naoto Yamaguchi, Junko Suda, Atsushi Iwama, Atsushi Hirao, Motohiro Hashiyama, Shin Ichi Aizawa, Toshio Suda

研究成果: Article査読

36 被引用数 (Scopus)

抄録

CHK/HYL is a non-receptor tyrosine kinase that belongs to CSK (C-terminal Src kinase) family, Northern blotting and RT-PCR analyses showed that CHK/HYL was expressed in large spectrum of hematopoietic cells except for erythroid cells and brain. To explore the function of CHK/HYL in hematopoietic cells, we generated CHK/HYL deficient mice. The mutant mice were apparently normal and fertile, while CSK knockout mice died until E11.5 from a defect in the neural tube formation. Hematological observations including blood counts and FAGS analysis showed no significant abnormalities in CHK/HYL mutant mice. CHK/HYL did not affect the activity of Src, Hck, and Fgr in cultured bone marrow cells, although CSK negatively regulates Src family kinases. These results suggest that CHK/HYL might not have the same function as CSK.

本文言語English
ページ(範囲)172-179
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
224
1
DOI
出版ステータスPublished - 1996 7月 5
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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