Analysis of peptides and proteins by temperature-responsive chromatographic system using N-isopropylacrylamide polymer-modified columns

Hideko Kanazawa, Kazuo Yamamoto, Yuki Kashiwase, Yoshikazu Matsushima, Nobuharu Takai, Akihiko Kikuchi, Yasuhisa Sakurai, Teruo Okano

研究成果: Article査読

79 被引用数 (Scopus)

抄録

A new method of HPLC using packing materials modified with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), was developed. Homogeneous PIPAAm polymer and its copolymer with butyl methacrylate (BMA) were synthesized and grafted to aminopropyl silica by activated ester-amine coupling and they were used as packing materials. The surface properties and functions of the stationary phases are controlled by external temperature. Isocratic elution by aqueous mobile phase alone is the basis for separation of peptides and protein. The separation of the mixture of three peptides, insulin chain A and B and β-endorphin fragment 1-27 was achieved by changing the column temperature with 0.9% NaCl aqueous solution as the sole eluent. Retention of peptides and proteins was controlled both by column temperature and by NaCl concentration in the aqueous mobile phases in this chromatographic system.

本文言語English
ページ(範囲)1545-1550
ページ数6
ジャーナルJournal of Pharmaceutical and Biomedical Analysis
15
9-10
DOI
出版ステータスPublished - 1997 6月

ASJC Scopus subject areas

  • 分析化学
  • 薬科学
  • 創薬
  • 分光学
  • 臨床生化学

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