Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

Ni Putu Desy Aryantini, Daisuke Kondoh, Keita Nishiyama, Yuji Yamamoto, Takao Mukai, I. Nengah Sujaya, Tadasu Urashima, Kenji Fukuda

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

本文言語English
論文番号fnx056
ジャーナルFEMS microbiology letters
364
6
DOI
出版ステータスPublished - 2017 3 1
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学
  • 遺伝学

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