Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

Takuma Fujii, Miyuki Saito, Takashi Iwata, Nobumaru Hirao, Hiroshi Nishio, Akiko Ohno, Katsumi Tsukazaki, Makio Mukai, Kaori Kameyama, Daisuke Aoki

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular-biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

本文言語English
ページ(範囲)545-555
ページ数11
ジャーナルVirchows Archiv
453
6
DOI
出版ステータスPublished - 2008 12

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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