@article{2a16a846eb5944f0a5a6d1d6a3bcee9f,
title = "Angptl 4 deficiency improves lipid metabolism, suppresses foam cell formation and protects against atherosclerosis",
abstract = "Angiopoietin-like protein family 4 (Angptl 4) has been shown to regulate lipoprotein metabolism through the inhibition of lipoprotein lipase (LPL). We generated ApoE-/-Angptl 4-/- mice to study the effect of Angptl 4 deficiency on lipid metabolism and atherosclerosis. Fasting and postolive oil-loaded triglyceride (TG) levels were largely decreased in ApoE-/-Angptl 4-/- mice compared with and ApoE-/-Angptl 4+/+ mice. There was a significant (75 ± 12%) reduction in atherosclerotic lesion size in ApoE-/-Angptl 4-/- mice compared with ApoE-/- Angptl 4+/+ mice. Peritoneal macrophages, isolated from Angptl 4-/- mice to investigate the foam cell formation, showed a significant decrease in newly synthesized cholesteryl ester (CE) accumulation induced by acetyl low-density lipoprotein (acLDL) compared with those from Angptl 4+/+ mice. Thus, genetic knockout of Angptl 4 protects ApoE-/- mice against development and progression of atherosclerosis and strongly suppresses the ability of the macrophages to become foam cells in vitro.",
keywords = "Angptl 4, Apolipoprotein E, Atherosclerosis, Foam cell formation, Foam cells, Knockout mice, Lipid metabolism, Lipoprotein lipase, Macrophage, Oxidized low-density lipoprotein, Postprandial hypertriglyceridemia",
author = "Hironori Adachi and Yukio Fujiwara and Tatsuya Kondo and Takeshi Nishikawa and Rei Ogawa and Takeshi Matsumura and Norio Ishii and Ryoji Nagai and Keishi Miyata and Mitsuhisa Tabata and Hiroyuki Motoshima and Noboru Furukawa and Kaku Tsuruzoe and Junji Kawashima and Motohiro Takeya and Shizuya Yamashita and Koh, {Gou Young} and Andras Nagy and Toshio Suda and Yuichi Oike and Eiichi Araki",
note = "Funding Information: We appreciate the helpful advice and assistance of Mr. Kenshi Ichinose in the Department of Metabolic Medicine of Medical and Pharmaceutical Sciences, Kumamoto University. RT-PCR primers for Angptl 4 were provided by Kunio Yasunaga (Department of Cardiology, Astellas Pharmaceutical Co. Ltd., Tsukuba, Japan). This work was supported by a grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science, Japan (Nos. 16046219 and 20390259 to Eiichi Araki), a grant from the Ministry of Education, Science, Sports and Culture of Japan (No. 19591058 to Tatsuya Kondo) and a grant from Suzuken Memorial Foundation (to Tatsuya Kondo).",
year = "2009",
month = feb,
day = "20",
doi = "10.1016/j.bbrc.2008.12.018",
language = "English",
volume = "379",
pages = "806--811",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",
}