Anisotropic spherical collagen microparticles for confined 3D cell culture system with spatially designed microenvironment

Satoru Yoshida, Masahiro Takinoue, Hiroaki Onoe

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

This paper describes anisotropic spherical collagen hydrogel microparticles for confined 3D cell culture system for the first time. We fabricated uniform-sized collagen microparticles simply by ejecting micro-droplets containing collagen pre-gel solution and sodium alginate into calcium chloride solution under high centrifugal gravity: we used calcium alginate hydrogel as a «sacrificial molecular template» for the gelation of pure collagen microparticles. Using our collagen microparticles, we successfully demonstrated in encapsulating differently labeled cells in each hemisphere at high viability and in culturing the cells inside the microparticles. Our anisotropic collagen microparticles could be powerful tools to co-culture multiple cells in different ECMs and to control differentiation of stem cells in an anisotropic microenvironment at single-particles with massive and quantitative analyses.

本文言語English
ホスト出版物のタイトルMEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
出版社Institute of Electrical and Electronics Engineers Inc.
ページ263-266
ページ数4
2016-February
ISBN(電子版)9781509019731
DOI
出版ステータスPublished - 2016 2月 26
イベント29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 - Shanghai, China
継続期間: 2016 1月 242016 1月 28

Other

Other29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
国/地域China
CityShanghai
Period16/1/2416/1/28

ASJC Scopus subject areas

  • 電子工学および電気工学
  • 機械工学
  • 凝縮系物理学
  • 電子材料、光学材料、および磁性材料

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