Anti-U5 small nuclear ribonucleoprotein (snRNP) antibodies: A rare anti-U snRNP specificity

Yutaka Okano, Ira N. Targoff, Chester V. Oddis, Takao Fujii, Masataka Kuwana, Kensei Tsuzaka, Michito Hirakata, Tsuneyo Mimori, Joe Craft, Thomas A. Medsger

研究成果: Article査読

19 被引用数 (Scopus)


We surveyed for autoantibodies to the U small nuclear ribonucleoproteins (snRNPs) in sera from 1171 patients with various connective tissue diseases by immunoprecipitation assay. We found serum, termed LaJ, which principally recognized the U5 snRNP from one patient with systemic sclerosis-polymyositis overlap syndrome. Anti-LaJ serum immunoprecipitated predominantly U5 snRNA from a 32PO4-labeled HeLa cell extract and at least five U5-specific proteins as well as the Sm core proteins from a [35S]methionine-labeled extract. Anti-LaJ serum immunoprecipitated both U5 snRNA and these five proteins in identical fractions at 15-20S by parallel sucrose gradient, suggesting physical association of these two components. Thus, we concluded that anti-LaJ serum contained antibody specific to the U5 snRNP. Antibodies specific to U5 snRNP were found in a single serum; in contrast, the prevalence of anti-U1 snRNP and anti-Sm antibody was considerably higher. Thus, the specific proteins of the U5 snRNB are rare targets for autoantibodies in patients with connective tissue disease.

ジャーナルClinical Immunology and Immunopathology
出版ステータスPublished - 1996 10月

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 病理学および法医学
  • 免疫学


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