TY - JOUR
T1 - Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots
AU - Matsushita, Hirohisa
AU - Kuwabara, Hiroaki
AU - Ishikawa, Satoko
AU - Mochizuki, Masataka
PY - 2005
Y1 - 2005
N2 - The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.
AB - The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.
KW - Apoptosis
KW - CCRF-HSB-2
KW - Chlorophytum comosum
UR - http://www.scopus.com/inward/record.url?scp=21144440555&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21144440555&partnerID=8YFLogxK
U2 - 10.1248/jhs.51.341
DO - 10.1248/jhs.51.341
M3 - Article
AN - SCOPUS:21144440555
SN - 1344-9702
VL - 51
SP - 341
EP - 345
JO - Journal of Health Science
JF - Journal of Health Science
IS - 3
ER -