An age is relatively important information in the various features which are shown human from face. It is possible to apply it to an amusement and an aesthetic surgery by analyzing the appearance-age feature which is used for age perception in a facial region. In this paper, we analyze facial feature of appearance-age based on age perception. In order to construct the appearance-age database, age estimation experiment is performed to the HOIP facial database by 15 subjects. The appearance-age feature used potentially when human performs age estimation is decided by using the Genetic Algorithms and the Neural Network. By using the proposed method, facial features that influenced on the appearance-age in each gender was confirmed. Moreover, it is suggested that that appearance-age feature which is used for age estimation is different in each generation when human performs age estimation.