Application of cationic latex particles for protein separation

Yoshiteru Sumi, Toshifumi Shiroya, Keiji Fujimoto, Tadashi Wada, Hiroshi Handa, Haruma Kawaguchi

研究成果: Article

24 引用 (Scopus)

抄録

We have prepared cationic latex particles with amino groups on their surface. The particles were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer, to which hexamethylenediamine was immobilized by means of coupling with epoxy groups. The zeta potential of the particles was approximately 30 mV and the isoelectric point was 10.5. The latex particles preferentially adsorbed acidic proteins through an electrostatic interaction. We compared the capacity and selectivity in adsorption of acidic proteins on the cationic particles and on diethylaminoethyl (DEAE)-Sepharose gel, which has been conventionally used. The latex particles were twenty times more efficient in adsorption of proteins than DEAE-Sepharose gel. Acidic proteins encoded by the adenovirus Ela gene were selectively separated from crude cell extracts using the cationic latex particles.

元の言語English
ページ(範囲)419-427
ページ数9
ジャーナルColloids and Surfaces B: Biointerfaces
2
発行部数4
DOI
出版物ステータスPublished - 1994 5 9

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Latex
latex
Latexes
Microspheres
Particles (particulate matter)
proteins
Proteins
1,6-diaminohexane
Sepharose
Adsorption
Gels
Polystyrenes
Isoelectric Point
Zeta potential
Coulomb interactions
Cell Extracts
Complex Mixtures
Static Electricity
Adenoviridae
adenoviruses

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

これを引用

Application of cationic latex particles for protein separation. / Sumi, Yoshiteru; Shiroya, Toshifumi; Fujimoto, Keiji; Wada, Tadashi; Handa, Hiroshi; Kawaguchi, Haruma.

:: Colloids and Surfaces B: Biointerfaces, 巻 2, 番号 4, 09.05.1994, p. 419-427.

研究成果: Article

Sumi, Yoshiteru ; Shiroya, Toshifumi ; Fujimoto, Keiji ; Wada, Tadashi ; Handa, Hiroshi ; Kawaguchi, Haruma. / Application of cationic latex particles for protein separation. :: Colloids and Surfaces B: Biointerfaces. 1994 ; 巻 2, 番号 4. pp. 419-427.
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