Application of cationic latex particles for protein separation

Yoshiteru Sumi, Toshifumi Shiroya, Keiji Fujimoto, Tadashi Wada, Hiroshi Handa, Haruma Kawaguchi

研究成果: Article査読

24 被引用数 (Scopus)

抄録

We have prepared cationic latex particles with amino groups on their surface. The particles were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer, to which hexamethylenediamine was immobilized by means of coupling with epoxy groups. The zeta potential of the particles was approximately 30 mV and the isoelectric point was 10.5. The latex particles preferentially adsorbed acidic proteins through an electrostatic interaction. We compared the capacity and selectivity in adsorption of acidic proteins on the cationic particles and on diethylaminoethyl (DEAE)-Sepharose gel, which has been conventionally used. The latex particles were twenty times more efficient in adsorption of proteins than DEAE-Sepharose gel. Acidic proteins encoded by the adenovirus Ela gene were selectively separated from crude cell extracts using the cationic latex particles.

本文言語English
ページ(範囲)419-427
ページ数9
ジャーナルColloids and Surfaces B: Biointerfaces
2
4
DOI
出版ステータスPublished - 1994 5月 9

ASJC Scopus subject areas

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学

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