Polypeptide-bound porphyrinatoiron(III) effectively catalyzed the N, N coupling reaction of N-methylaniline in the presence of NaBH4/O2/tetramethyl-ammonium hydroxide or cumenehydroperoxide, whereas the corresponding non-bound monomer porphyrinatoiron(III) had little catalytic activity. Rat liver microsomes also catalyzed the N, N-coupling which was inhibited by cytochrome P-450 specific inhibitors, SKF-525A and metyrapone. The polypeptide-bound porphinatoiron(III) appears to be an excellent cytochrome P450 model for drug metabolism studies.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版物ステータス||Published - 1993 3 15|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology