Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants.

Zhiwei Cai, Yoshiro Sohma, Silvia G. Bompadre, David N. Sheppard, Tzyh Chang Hwang

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants.

本文言語English
ページ(範囲)419-441
ページ数23
ジャーナルMethods in molecular biology (Clifton, N.J.)
741
DOI
出版ステータスPublished - 2011

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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