TY - JOUR
T1 - Application of high-resolution single-channel recording to functional studies of cystic fibrosis mutants.
AU - Cai, Zhiwei
AU - Sohma, Yoshiro
AU - Bompadre, Silvia G.
AU - Sheppard, David N.
AU - Hwang, Tzyh Chang
PY - 2011
Y1 - 2011
N2 - The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants.
AB - The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants.
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U2 - 10.1007/978-1-61779-117-8_27
DO - 10.1007/978-1-61779-117-8_27
M3 - Article
C2 - 21594800
AN - SCOPUS:80052338842
VL - 741
SP - 419
EP - 441
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -