Application of PCR for Mycoplasma pneumoniae detection in children with community-acquired pneumonia

Miyuki Morozumi, Keiko Hasegawa, Naoko Chiba, Satoshi Iwata, Naohisa Kawamura, Haruo Kuroki, Takeshi Tajima, Kimiko Ubukata

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Between April 2002 and March 2003, to detect Mycoplasma pneumoniae by polymerase chain reaction (PCR), a primer set designed for the 16S rRNA gene was used to examine clinical samples from 369 children with community-acquired pneumonia. Samples were collected from 12 Japanese institutions participating in a study group concerning acute respiratory infectious diseases. The sensitivity of primers - 2 CFU per reaction tube, using M. pneumoniae M129, a standard strain - was calculated to represent 1.1 × 103 M. pneumoniae organisms adherent to the tip of the swab used to collect clinical samples. Results for PCR were obtained within 2.6 h. Cases identified by PCR, cultures, and serologic tests were 68 (18.4%), 53 (14.4%), and 76 (20.6%) respectively. Among 57 PCR-positive patients tested serologically, 56 showed a significant elevation or rise in antibody titer. PCR positivity was high among patients prescribed β-lactam antibiotics (86.7%) or no antibiotic (87.0%) before PCR analysis, but was low among patients receiving macrolides, new quinolones, or tetracyclines (37.5%). We concluded that the PCR constructed by us had a high probability for confirming a diagnosis of M. pneumoniae pneumonia and for guiding antibiotic choice for patients not yet treated.

本文言語English
ページ(範囲)274-279
ページ数6
ジャーナルJournal of Infection and Chemotherapy
10
5
DOI
出版ステータスPublished - 2004 10

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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