Application of recombination transfer to the cognate Bacillus subtilis genome.

Satoshi Tomita, Kenji Tsuge, Y. Kikuchi, Mitsuhiro Itaya

研究成果: Article査読

抄録

Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

本文言語English
ページ(範囲)295-296
ページ数2
ジャーナルNucleic acids research. Supplement (2001)
3
DOI
出版ステータスPublished - 2003

ASJC Scopus subject areas

  • 医学(全般)

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