Aquaporins in rat pancreatic interlobular ducts

Shigeru B.H. Ko, Satoru Naruse, Motoji Kitagawa, Hiroshi Ishiguro, Sonoko Furuya, Nobumasa Mizuno, Youxue Wang, Toshiyuki Yoshikawa, Atsushi Suzuki, Shoko Shimano, Tetsuo Hayakawa

研究成果: Article査読

46 被引用数 (Scopus)


The aquaporin (AQP) family of water channels is distributed ubiquitously in many epithelia and plays a fundamental role in transmembrane water transport. The aim of this study is to identify the water transport pathway in pancreatic duct cells where most of the HCO3--rich fluid originates. Using digital videomicroscopy, we measured the osmotic water permeability (Pf) of pancreatic duct epithelium by exposing isolated rat interlobular ducts to the hypotonic solution (145 mosM). To identify mRNA and protein of AQPs expressed in duct cells, we conducted RT-PCR analysis and immunohistochemistry of the isolated duct and pancreas. The calculated Pf (160-230 μm/s) of the isolated ducts was significantly reduced to 16-35 μm/s by 80-90% with either basolateral or luminal applications of HgCl2. Fluid secretion evoked by secretin was almost completely abolished by a basolateral or luminal application of HgCl2. A large amount of AQP1 and small amounts of AQP5 transcripts were detected in the isolated duct cells by RT-PCR. AQP1, but not AQP5, immunoreactivity was present in both luminal and basolateral membranes of the interlobular duct cells. Mercury-sensitive water channels are present in both luminal and basolateral membranes of rat pancreatic ducts. AQP1 of the known AQPs appears to be the main water pathway in interlobular ducts.

ジャーナルAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
2 45-2
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • 生理学
  • 肝臓学
  • 消化器病学
  • 生理学(医学)


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