Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature

Masayoshi Nakasako, Mikio Kataoka, Fumio Tokunaga

研究成果: Article査読

19 被引用数 (Scopus)

抄録

The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.

本文言語English
ページ(範囲)211-214
ページ数4
ジャーナルFEBS Letters
254
1-2
DOI
出版ステータスPublished - 1989 8 28
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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