TY - JOUR
T1 - Arginine vasopressin inhibits fluid secretion in guinea pig pancreatic duct cells
AU - Ko, S. B.H.
AU - Naruse, S.
AU - Kitagawa, M.
AU - Ishiguro, H.
AU - Murakami, M.
AU - Hayakawa, T.
PY - 1999/7
Y1 - 1999/7
N2 - The effects of arginine vasopressin (AVP) on pancreatic ductal secretion were studied in guinea pigs. In the isolated vascularly perfused pancreas, AVP reduced secretin-stimulated fluid secretion and increased the vascular resistance when the perfusion rate was held constant. In the isolated interlobular duct segments, AVP inhibited secretin-stimulated fluid secretion, indicating the direct inhibitory action of AVP on the duct cells. AVP affected neither the basal nor the secretin-induced cAMP productions, suggesting that AVP inhibits the fluid secretion at a point distal to the production of cAMP. AVP increased intracellular Ca2+ concentration ([Ca2+](i)) in the absence of extracellular Ca2+. When [Ca2+](i) was elevated by the application of thapsigargin, AVP caused a rapid decrease in [Ca2+](i). AVP seems to activate both Ca2+ release from intracellular stores and Ca2+ efflux across the plasma membrane, but its relation to the inhibition of fluid secretion remains to be clarified. It is concluded that AVP directly inhibits secretin-stimulated ductal fluid secretion in the guinea pig pancreas.
AB - The effects of arginine vasopressin (AVP) on pancreatic ductal secretion were studied in guinea pigs. In the isolated vascularly perfused pancreas, AVP reduced secretin-stimulated fluid secretion and increased the vascular resistance when the perfusion rate was held constant. In the isolated interlobular duct segments, AVP inhibited secretin-stimulated fluid secretion, indicating the direct inhibitory action of AVP on the duct cells. AVP affected neither the basal nor the secretin-induced cAMP productions, suggesting that AVP inhibits the fluid secretion at a point distal to the production of cAMP. AVP increased intracellular Ca2+ concentration ([Ca2+](i)) in the absence of extracellular Ca2+. When [Ca2+](i) was elevated by the application of thapsigargin, AVP caused a rapid decrease in [Ca2+](i). AVP seems to activate both Ca2+ release from intracellular stores and Ca2+ efflux across the plasma membrane, but its relation to the inhibition of fluid secretion remains to be clarified. It is concluded that AVP directly inhibits secretin-stimulated ductal fluid secretion in the guinea pig pancreas.
KW - Adenosine 3',5'-cyclic monophosphate
KW - Intracellular calcium concentration
KW - Isolated interlobular duct segment
KW - Isolated vascularly perfused pancreas
KW - Luminal volume
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U2 - 10.1152/ajpgi.1999.277.1.g48
DO - 10.1152/ajpgi.1999.277.1.g48
M3 - Article
C2 - 10409150
AN - SCOPUS:0032791999
VL - 277
SP - G48-G54
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 1 40-1
ER -