@article{a7a5656ef22e4e7291e4cac8425ea875,
title = "Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos",
abstract = "The small GTPase Arl8b localizes primarily to lysosomes and is involved in lysosomal motility and fusion. Here, we show that Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm (VYSE), an apical cell layer of the visceral yolk sac, of mouse embryos. The VYSE actively takes up maternal materials from uterine fluid and degrades them in lysosomes to provide breakdown products to the embryo. Arl8b gene-trap mice (Arl8b-/-) displayed decreased early embryo body size. The Arl8b-/- VYSE exhibited defective endocytic trafficking to the lysosome and accumulation of maternal proteins such as albumin and immunoglobulin G in late endocytic organelles. Furthermore, Transthyretin-Cre;Arl8bflox/flox mice in which Arl8b was ablated specifically in the VYSE also showed decreased embryo body size, defects in trafficking to the lysosome and reduction of the free amino acid level in the embryos. Taken together, these results suggest that Arl8b mediates lysosomal degradation of maternal proteins in the VYSE, thereby contributing to mouse embryonic development.",
keywords = "Embryogenesis, Endocytosis, Lysosome, Metabolomics, Small GTPase",
author = "Miho Oka and Keisuke Hashimoto and Yoshifumi Yamaguchi and Saitoh, {Shin Ichiro} and Yuki Sugiura and Yuji Motoi and Kurara Honda and Yorifumi Kikko and Shinya Ohata and Makoto Suematsu and Masayuki Miura and Kensuke Miyake and Toshiaki Katada and Kenji Kontani",
note = "Funding Information: This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (grant numbers 26110005 to Y.Y. and 23113706 to K.K.); AMED-CREST, AMED (grant number 16GM0610004H0005 to M.M.); the Japan Society for the Promotion of Science (JSPS) (grant numbers 14J12392 to K.H.; 26293083 to S.S.; 16H06163 to S.O.; 16H06385 to M.M.; 23229001 to T.K., and 23370083 and 26291038 to K.K.); Mochida Memorial Foundation for Medical and Pharmaceutical Research to S.O.; Yasuda Memorial Medical Foundation to S.O.; and Takeda Science Foundation to S.S. Publisher Copyright: {\textcopyright} 2017.",
year = "2017",
doi = "10.1242/jcs.200519",
language = "English",
volume = "130",
pages = "3568--3577",
journal = "The Quarterly journal of microscopical science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "20",
}