Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation

Haruna Yamashiro, Mayu Negishi, Tatsuki Kinoshita, Hirotsugu Ishizu, Hitoshi Ohtani, Mikiko C. Siomi

研究成果: Article

1 引用 (Scopus)

抜粋

Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.

元の言語English
記事番号e48769
ジャーナルEMBO Reports
21
発行部数2
DOI
出版物ステータスPublished - 2020 2 5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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