Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

Akira Kono, Setsuro Sugata, Yasuhiro Hara, Mutsuko Tanaka, Yoshiharu Karube, Yoshikazu Matsushima

研究成果: Article査読

3 被引用数 (Scopus)

抄録

A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5’-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

本文言語English
ページ(範囲)1666-1669
ページ数4
ジャーナルChemical and Pharmaceutical Bulletin
31
5
DOI
出版ステータスPublished - 1983 1 1

ASJC Scopus subject areas

  • 化学 (全般)
  • 創薬

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