Assay of uridine diphosphate glucuronosyltransferase by high-pressure liquid chromatography

Michio Matsui, Fusako Nagai

研究成果: Article

13 引用 (Scopus)

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A new and simple method for the determination of rat liver microsomal uridine diphosphate glucuronosyltransferase activity toward 4-nitrophenol, phenolphthalein, and testosterone has been developed with the use of high-pressure liquid chromatography (hplc). After incubation of the substrate with microsomal fractions in the presence of uridine diphosphate glucuronic acid, the reaction is stopped by heating, mixed with methanol, and centrifuged to give the supernatant, which is analyzed directly by hplc. The unreacted substrate as well as its glucuronide can be quantitated from the peak-height method. When the pure glucuronide is not available, the standard curve for the glucuronide is obtained from the enzymatically produced glucuronide.

元の言語English
ページ(範囲)141-146
ページ数6
ジャーナルAnalytical Biochemistry
105
発行部数1
DOI
出版物ステータスPublished - 1980 6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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