Assay of uridine diphosphate glucuronosyltransferase by high-pressure liquid chromatography

Michio Matsui, Fusako Nagai

研究成果: Article査読

13 被引用数 (Scopus)

抄録

A new and simple method for the determination of rat liver microsomal uridine diphosphate glucuronosyltransferase activity toward 4-nitrophenol, phenolphthalein, and testosterone has been developed with the use of high-pressure liquid chromatography (hplc). After incubation of the substrate with microsomal fractions in the presence of uridine diphosphate glucuronic acid, the reaction is stopped by heating, mixed with methanol, and centrifuged to give the supernatant, which is analyzed directly by hplc. The unreacted substrate as well as its glucuronide can be quantitated from the peak-height method. When the pure glucuronide is not available, the standard curve for the glucuronide is obtained from the enzymatically produced glucuronide.

本文言語English
ページ(範囲)141-146
ページ数6
ジャーナルAnalytical Biochemistry
105
1
DOI
出版ステータスPublished - 1980 6

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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