TY - JOUR
T1 - Associations between Loss of ARID1A Expression and Clinicopathologic and Genetic Variables in T1 Early Colorectal Cancer
AU - Kishida, Yoshihiro
AU - Oishi, Takuma
AU - Sugino, Takashi
AU - Shiomi, Akio
AU - Urakami, Kenichi
AU - Kusuhara, Masatoshi
AU - Yamaguchi, Ken
AU - Kitagawa, Yuko
AU - Ono, Hiroyuki
N1 - Funding Information:
Acknowledgments: We thank Mr. Koji Muramatsu (medical laboratory scientist) and the members of the Shizuoka Cancer Center Hospital and Research Institute for their support and valuable technical assistance. This work was supported by the Shizuoka Cancer Center.
Publisher Copyright:
© 2019 American Society for Clinical Pathology.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/9/9
Y1 - 2019/9/9
N2 - Objectives: To evaluate the relationships between adenine-thymine-rich interactive domain 1A (ARID1A) expression and the clinicopathologic features in T1 colorectal cancer (CRC) and to investigate whether the presence of ARID1A protein is related to genetic changes. Methods: We retrospectively studied 219 surgically resected T1 CRCs. ARID1A expression was assessed by immunohistochemical methods, and the correlation between ARID1A expression and clinicopathologic features was evaluated. The relationship between ARID1A expression and 409 cancer-related gene mutations was also evaluated using next-generation sequencing (NGS). Results: Immunohistochemical staining indicated negative ARID1A expression in 4.6%. Loss of ARID1A expression was significantly associated with younger age, lymphatic invasion, and lymph node metastasis (LNM). NGS showed that PKHD1, RNF213, and MSH6 mutations were more frequent in ARID1A-negative tumors, whereas KRAS mutations were more common in ARID1A-positive tumors. Conclusions: In T1 CRC, negative ARID1A expression was correlated with early onset, lymphatic invasion, and LNM. Mutations in some cancer-related genes were possibly related with ARID1A expression.
AB - Objectives: To evaluate the relationships between adenine-thymine-rich interactive domain 1A (ARID1A) expression and the clinicopathologic features in T1 colorectal cancer (CRC) and to investigate whether the presence of ARID1A protein is related to genetic changes. Methods: We retrospectively studied 219 surgically resected T1 CRCs. ARID1A expression was assessed by immunohistochemical methods, and the correlation between ARID1A expression and clinicopathologic features was evaluated. The relationship between ARID1A expression and 409 cancer-related gene mutations was also evaluated using next-generation sequencing (NGS). Results: Immunohistochemical staining indicated negative ARID1A expression in 4.6%. Loss of ARID1A expression was significantly associated with younger age, lymphatic invasion, and lymph node metastasis (LNM). NGS showed that PKHD1, RNF213, and MSH6 mutations were more frequent in ARID1A-negative tumors, whereas KRAS mutations were more common in ARID1A-positive tumors. Conclusions: In T1 CRC, negative ARID1A expression was correlated with early onset, lymphatic invasion, and LNM. Mutations in some cancer-related genes were possibly related with ARID1A expression.
KW - ARID1A
KW - Early colorectal cancer
KW - Immunohistochemistry staining
KW - Next-generation sequencing
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U2 - 10.1093/ajcp/aqz062
DO - 10.1093/ajcp/aqz062
M3 - Article
C2 - 31263894
AN - SCOPUS:85071998070
VL - 152
SP - 463
EP - 470
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -
