Astrocytic lineage analysis by detection of GFAP promoter activity in vitro

Noriyuki Morita, Kensuke Nakahira, Hiroko Baba, Hiromi Akita, Tatsuro Kumada, Masaharu Ogawa, Kazunori Nakajima, Mitsuhiro Kawata, Katsuhiko Mikoshiba, Kazuhiro Ikenaka

研究成果: Article

10 引用 (Scopus)

抄録

To survey the emergence and onset of differentiation of the astrocytic lineage in the developing mouse cerebral wall, the promoter activity of a 2.5 kb 5'-flanking region of glial fibrillary acidic protein (GFAP) was measured in individual developing brain cells using a retrovirus-mediated gene transfer system. We identified precursors for astrocytes in primary culture of embryonic mouse cerebral wall cells by detection of GFAP promoter activity, which was detected approximately 3 days prior to the appearance of GFAP immunoreactivity. Since retroviruses only integrate into the chromosomes of actively proliferating cells, cells detected by this method should have been mitotically active at the time of retroviral infection on day 15 postfertilization (E15). Furthermore, we observed that cells activating GFAP promoter were located near the ventricular surface of cultured cerebral wall slices as a cluster of spherical cells. These results demonstrate that precursor cells for astrocytes exist within the germinative zone of developing cerebral wall, and that these cells are mitotically active on day E15, which is a late stage of neuronal production period in the mouse cerebral wall. The morphology, location and mitotic activity of these cells suggest that they are unlikely to be cells that have been transformed from radial glial cells.

元の言語English
ページ(範囲)210-218
ページ数9
ジャーナルDevelopmental Neuroscience
19
発行部数2
出版物ステータスPublished - 1997 3

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Glial Fibrillary Acidic Protein
Retroviridae
Astrocytes
Cell Wall
Ependymoglial Cells
In Vitro Techniques
5' Flanking Region
Chromosomes
Brain
Infection
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

Morita, N., Nakahira, K., Baba, H., Akita, H., Kumada, T., Ogawa, M., ... Ikenaka, K. (1997). Astrocytic lineage analysis by detection of GFAP promoter activity in vitro. Developmental Neuroscience, 19(2), 210-218.

Astrocytic lineage analysis by detection of GFAP promoter activity in vitro. / Morita, Noriyuki; Nakahira, Kensuke; Baba, Hiroko; Akita, Hiromi; Kumada, Tatsuro; Ogawa, Masaharu; Nakajima, Kazunori; Kawata, Mitsuhiro; Mikoshiba, Katsuhiko; Ikenaka, Kazuhiro.

:: Developmental Neuroscience, 巻 19, 番号 2, 03.1997, p. 210-218.

研究成果: Article

Morita, N, Nakahira, K, Baba, H, Akita, H, Kumada, T, Ogawa, M, Nakajima, K, Kawata, M, Mikoshiba, K & Ikenaka, K 1997, 'Astrocytic lineage analysis by detection of GFAP promoter activity in vitro', Developmental Neuroscience, 巻. 19, 番号 2, pp. 210-218.
Morita N, Nakahira K, Baba H, Akita H, Kumada T, Ogawa M その他. Astrocytic lineage analysis by detection of GFAP promoter activity in vitro. Developmental Neuroscience. 1997 3;19(2):210-218.
Morita, Noriyuki ; Nakahira, Kensuke ; Baba, Hiroko ; Akita, Hiromi ; Kumada, Tatsuro ; Ogawa, Masaharu ; Nakajima, Kazunori ; Kawata, Mitsuhiro ; Mikoshiba, Katsuhiko ; Ikenaka, Kazuhiro. / Astrocytic lineage analysis by detection of GFAP promoter activity in vitro. :: Developmental Neuroscience. 1997 ; 巻 19, 番号 2. pp. 210-218.
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