ATAC-seq method applied to embryonic germ cells and neural stem cells from mouse: Practical tips and modifications

Soichiro Yamanaka, Yusuke Kishi, Haruhiko Siomi

研究成果: Chapter

2 被引用数 (Scopus)

抄録

The chromatin state affects the level of transcription at the initial stage of gene expression. Therefore, it is important to determine chromatin accessibility in regions of the genome that contain regulatory elements. To this end, several methods have been developed. The ATAC-seq method outperforms the other methods in profiling chromatin accessibility for every single cell, and standardized library preparation kits are now commercially available. In this chapter, we describe the ATAC-seq method that we have applied to mouse embryonic germ cells as well as to mouse neural stem cells, which are relatively rare. We also developed new method to examine the changes in global accessibility during their developmental process using spike-in normalization with fly cells.

本文言語English
ホスト出版物のタイトルEpigenetics Methods
出版社Elsevier
ページ371-386
ページ数16
ISBN(電子版)9780128194140
DOI
出版ステータスPublished - 2020 1月 1

ASJC Scopus subject areas

  • 医学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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