Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm

Yasuyoshi Hase, Michiko Tatsuno, Takeyuki Nishi, Kosuke Kataoka, Yasuaki Kabe, Yuki Yamaguchi, Nobuaki Ozawa, Michiya Natori, Hiroshi Handa, Hajime Watanabe

研究成果: Article査読

43 被引用数 (Scopus)

抄録

Atrazine is a widely used triazine herbicide. Although controversy still exists, a number of recent studies have described its adverse effects on various animals including humans. Of particular interest is its effects on reproductive capacity. In this study, we investigated the mechanisms underlying the adverse effects of atrazine, with a focus on its effects on sperm. Here we show evidence that mitochondrial F1F0-ATP synthase is a molecular target of atrazine. A series of experiments with sperm and isolated mitochondria suggest that atrazine inhibits mitochondrial function through F1F0-ATP synthase. Moreover, affinity purification using atrazine as a ligand demonstrates that F1F0-ATP synthase is a major atrazine-binding protein in cells. The inhibitory activity against mitochondria and F1F0-ATP synthase is not limited to atrazine but is likely to be applicable to other triazine-based compounds. Thus, our findings may have wide relevance to pharmacology and toxicology.

本文言語English
ページ(範囲)66-72
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
366
1
DOI
出版ステータスPublished - 2008 2 1
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Atrazine binds to F<sub>1</sub>F<sub>0</sub>-ATP synthase and inhibits mitochondrial function in sperm」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル