The effect of α-human atrial natriuretic polypeptide (α-hANP) on the synthesis of prostaglandins was studied in isolated rat glomeruli. Glomeruli were isolated by a passive seiving technique according to the method of Misra and were incubated at 37°C for 60 min in the presence (Group II: 10-6 M, Group III: 10-5 M) or absence (Group I: control) of α-hANP. Furthermore, glomeruli were incubated with arachidonic acid (10-5, 10-4, and 10-3 M) and at the end of the incubation period trypan blue was added to the glomerular suspension. The presence of α-hANP (10-6 and 10-5 M) caused no significant difference in prostaglandin synthesis as compared with the control. On the other hand, arachidonic acid stimulated prostaglandin synthesis and the glomerular preparation was not stained by trypan blue, indicating that it remained viable. These results suggest that α-hANP does not directly affect the prostaglandin synthesis in isolated rat glomeruli.
|出版ステータス||Published - 1986|
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