Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Arai, Akira Sugawara, Narito Morii, Takayuki Yamada, Hiroshi Itoh, Shozo Shiono, Masashi Mukoyama, Kenji Obata, Hirofumi Yasue, Hiroaki Ohkubo, Shigetada Nakanishi, Hiroo Imura

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ventricle was 0.2 % of that in the atrium. The ANPmRNA level in the DCM ventricle increased to about 7 % of that in the corresponding atrium and was approximately 40 times higher than that in the control ventricle, although the ANPmRNA level in the DCM atrium was comparable to that in the control atrium. The total content of ANPmRNA in the DCM ventricle reached about 30 % of that in the corresponding atrium and was much the same as that in the control atrium. The ANP level in the DCM ventricle was approximately 1.0 μg/g and much higher than that in the control ventricle (0.02 μg/g).

本文言語English
ページ(範囲)211-217
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
148
1
DOI
出版ステータスPublished - 1987 10月 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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