Atrial natriuretic polypeptide in bovine adrenal medulla

Masashi Mukoyama, Kazuwa Nakao, Narito Morii, Shozo Shiono, Hiroshi Itoh, Akira Sugawara, Takayuki Yamada, Yoshihiko Saito, Hiroshi Arai, Hiroo Mura

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Two radioimmunoassays for α-human atrial natriuretic polypeptide (a-hANP) with different specificities were used to study the tissue level and the nature of α-hANP-like immunoreactivity in the bovine adrenal gland. A considerable amount of α-hANP-like immunoreactivity was detected in the adrenal medulla (90.8 ± 21.1 and 90.0 ± 23.1 ng/g with the two radioimmunoassays), while no detectable amount (<1.0 μg) was present in the cortex. Gel permeation chromatographic analysis showed that ANP in the medulla is composed of two components of α-hANP-like immunoreactivity with high and low molecular weights in the approximate ratio of 2:1, eluting at the elution positions of y-hANP and α-hANP, respectively. Reverse-phase high performance liquid chromatographic analysis revealed that α-hANP-like immunoreactivity with a low molecular weight in the medulla consists of two major components, which comigrate with synthetic α-hANP(5-28) and ahANP. When cultured bovine adrenal chrom'affin cells were incubated in the presence of nicotine (10-5 M), α-hANP-like immunoreactivity was released into the medium concomitantly with catecholamines from chromaffin cells. These findings indicate that a discrete ANP system is present in the adrenal medulla and that ANP is cosecreted with catecholamines from chromaffin cells, suggesting the possible involvement of ANP in the adrenomedullary function.

本文言語English
ページ(範囲)692-696
ページ数5
ジャーナルHypertension
11
6
DOI
出版ステータスPublished - 1988 6
外部発表はい

ASJC Scopus subject areas

  • 内科学

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