Using a radioimmunoassay for α-rat atrial natriuretic polypeptide (α-rANP), tissue levels of α-rANP-like immunoreactivity (-LI) in the rat spinal cord and autonomic ganglia were investigated. The α-rANP-LI level was higher in the more caudal parts of the spinal cord and the highest in the sacral spinal cord. α-rANP-LI was also detected in the superior cervical and coeliac ganglia. Gel permeation chromatographic analysis showed that the major peak of α-rANP-LI in the spinal cord was a low molecular weight form co-eluted with synthetic α-rANP. Reverse-phase high performance liquid chromatographic analysis revealed that α-rANP-LI with a low molecular weight in the spinal cord consisted of several components, two major components of which co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), whereas little immunoreactivity was eluted at the position of α-rANP. These findings suggest the involvement of ANP in the function of the spinal cord and autonomic nervous system.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||Published - 1987 5月 29|
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