Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells

Toru Hirota, Naoko Kunitoku, Takashi Sasayama, Tomotoshi Marumoto, Dongwei Zhang, Masayuki Nitta, Katsuyoshi Hatakeyama, Hideyuki Saya

研究成果: Article査読

513 被引用数 (Scopus)

抄録

Aurora family kinases contribute to regulation of mitosis. Using RNA interference in synchronized HeLa cells, we now show that Aurora-A is required for mitotic entry. We found that initial activation of Aurora-A in late G2 phase of the cell cycle is essential for recruitment of the cyclin B1-Cdk1 complex to centrosomes, where it becomes activated and commits cells to mitosis. A two-hybrid screen identified the LIM protein Ajuba as an Aurora-A binding protein. Ajuba and Aurora-A interact in mitotic cells and become phosphorylated as they do so. In vitro analyses revealed that Ajuba induces the autophosphorylation and consequent activation of Aurora-A. Depletion of Ajuba prevented activation of Aurora-A at centrosomes in late G2 phase and inhibited mitotic entry. Overall, our data suggest that Ajuba is an essential activator of Aurora-A in mitotic commitment.

本文言語English
ページ(範囲)585-598
ページ数14
ジャーナルCell
114
5
DOI
出版ステータスPublished - 2003 9 5
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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