Automated drug screening system for ion channel proteins

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, H. Sasaki, K. Kamiya, N. Miki, T. Ide, S. Takeuchi

研究成果: Conference contribution

抄録

This paper describes an automated drug screening system for Ca 2+-activated K+ channel proteins (BK channel). BK channels are pharmacological targets for the treatment of stroke. We have been developing multiple lipid bilayer membranes (BLMs) system for reconstituting the membrane proteins [1]. In this study, the osmotic pressure in the proteoliposomes was controlled to induce the vesicle fusion. As a result, we succeeded in simultaneous reconstitution of BK channels in the BLMs array using an injection robot (Fig. 1). Moreover, the channel inhibition behavior was examined using a K+ channel blocker. Since our method is applicable for multi-type of proteoliposomes, we believe that the system can be used as a high-throughput screening (HTS) system for membrane-protein-based drug discovery.

本文言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ76-78
ページ数3
出版ステータスPublished - 2011
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10 22011 10 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域United States
CitySeattle, WA
Period11/10/211/10/6

ASJC Scopus subject areas

  • 制御およびシステム工学

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