Automated parallel recordings of topologically identified single ion channels

Ryuji Kawano, Yutaro Tsuji, Koji Sato, Toshihisa Osaki, Koki Kamiya, Minako Hirano, Toru Ide, Norihisa Miki, Shoji Takeuchi

研究成果: Article

72 引用 (Scopus)

抄録

Although ion channels are attractive targets for drug discovery, the systematic screening of ion channel-targeted drugs remains challenging. To facilitate automated single ion-channel recordings for the analysis of drug interactions with the intra-and extracellular domain, we have developed a parallel recording methodology using artificial cell membranes. The use of stable lipid bilayer formation in droplet chamber arrays facilitated automated, parallel, single-channel recording from reconstituted native and mutated ion channels. Using this system, several types of ion channels, including mutated forms, were characterised by determining the protein orientation. In addition, we provide evidence that both intra-and extracellular amyloid-beta fragments directly inhibit the channel open probability of the hBK channel. This automated methodology provides a high-throughput drug screening system for the targeting of ion channels and a data-intensive analysis technique for studying ion channel gating mechanisms.

元の言語English
記事番号1995
ジャーナルScientific Reports
3
DOI
出版物ステータスPublished - 2013

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Ion Channels
Ion Channel Gating
Artificial Cells
Artificial Membranes
Preclinical Drug Evaluations
Lipid Bilayers
Drug Discovery
Drug Interactions
Amyloid
Cell Membrane
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • General
  • Medicine(all)

これを引用

Kawano, R., Tsuji, Y., Sato, K., Osaki, T., Kamiya, K., Hirano, M., ... Takeuchi, S. (2013). Automated parallel recordings of topologically identified single ion channels. Scientific Reports, 3, [1995]. https://doi.org/10.1038/srep01995

Automated parallel recordings of topologically identified single ion channels. / Kawano, Ryuji; Tsuji, Yutaro; Sato, Koji; Osaki, Toshihisa; Kamiya, Koki; Hirano, Minako; Ide, Toru; Miki, Norihisa; Takeuchi, Shoji.

:: Scientific Reports, 巻 3, 1995, 2013.

研究成果: Article

Kawano, R, Tsuji, Y, Sato, K, Osaki, T, Kamiya, K, Hirano, M, Ide, T, Miki, N & Takeuchi, S 2013, 'Automated parallel recordings of topologically identified single ion channels', Scientific Reports, 巻. 3, 1995. https://doi.org/10.1038/srep01995
Kawano, Ryuji ; Tsuji, Yutaro ; Sato, Koji ; Osaki, Toshihisa ; Kamiya, Koki ; Hirano, Minako ; Ide, Toru ; Miki, Norihisa ; Takeuchi, Shoji. / Automated parallel recordings of topologically identified single ion channels. :: Scientific Reports. 2013 ; 巻 3.
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