Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance

Adam M. Corrigan, Roshan L. Shrestha, Ihsan Zulkipli, Noriko Hiroi, Yingjun Liu, Naoka Tamura, Bing Yang, Jessica Patel, Akira Funahashi, Athene Donald, Viji M. Draviam

研究成果: Article

16 引用 (Scopus)

抜粋

Spindle orientation defines the plane of cell division and, thereby, the spatial position of all daughter cells. Here, we develop a live cell microscopy-based methodology to extract spindle movements in human epithelial cell lines and study how spindles are brought to a pre-defined orientation. We show that spindles undergo two distinct regimes of movements. Spindles are first actively rotated toward the cells' long-axis and then maintained along this pre-defined axis. By quantifying spindle movements in cells depleted of LGN, we show that the first regime of rotational movements requires LGN that recruits cortical dynein. In contrast, the second regime of movements that maintains spindle orientation does not require LGN, but is sensitive to 2ME2 that suppresses microtubule dynamics. Our study sheds first insight into spatially defined spindle movement regimes in human cells, and supports the presence of LGN and dynein independent cortical anchors for astral microtubules.

元の言語English
ページ(範囲)2643-2655
ページ数13
ジャーナルCell Cycle
12
発行部数16
DOI
出版物ステータスPublished - 2013 8 15

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • これを引用

    Corrigan, A. M., Shrestha, R. L., Zulkipli, I., Hiroi, N., Liu, Y., Tamura, N., Yang, B., Patel, J., Funahashi, A., Donald, A., & Draviam, V. M. (2013). Automated tracking of mitotic spindle pole positions shows that LGN is required for spindle rotation but not orientation maintenance. Cell Cycle, 12(16), 2643-2655. https://doi.org/10.4161/cc.25671