Automatic analysis for neuron by confocal laser scanning microscope

Kouhei Satou, Yoshimitu Aoki, Nobuko Mataga, Takao K. Hensh, Katuhiko Taki

研究成果: Conference article

1 引用 (Scopus)

抜粋

The aim of this study is to develop a system that recognizes both the macro- and microscopic configurations of nerve cells and automatically performs the necessary 3-D measurements and functional classification of spines. The acquisition of 3-D images of cranial nerves has been enabled by the use of a confocal laser scanning microscope, although the highly accurate 3-D measurements of the microscopic structures of cranial nerves and their classification based on their configurations have not yet been accomplished. In this study, in order to obtain highly accurate measurements of the microscopic structures of cranial nerves, existing positions of spines were predicted by the 2-D image processing of tomographic images. Next, based on the positions that were predicted on the 2-D images, the positions and configurations of the spines were determined more accurately by 3-D image processing of the volume data. We report the successful construction of an automatic analysis system that uses a coarse-to-fine technique to analyze the microscopic structures of cranial nerves with high speed and accuracy by combining 2-D and 3-D image analyses.

元の言語English
記事番号60510A
ジャーナルProceedings of SPIE - The International Society for Optical Engineering
6051
DOI
出版物ステータスPublished - 2005 12 1
外部発表Yes
イベントOptomechatronic Machine Vision - Sappora, Japan
継続期間: 2005 12 52005 12 7

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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