Automatic generation system of cell-sized liposomes

Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

研究成果: Article

抄録

Artificial cell membranes such as liposomes and lipid vesicles have been developed using microfluidic technologies. These technologies have advantages for generating cell-sized liposomes, including a monodispersed and asymmetric lipid composition. Cell-sized asymmetric liposomes with various combinations of asymmetric lipid leaflets are sequentially produced using an improved pulsed-jetting device with the six wells arranged on a circular rotation table by manual manipulation. In this study, we reveal optimal parameters of rotation speed and jetting timing for the cell-sized liposome formation using an automatic sequential liposome generation system driven by a stepping motor. First, we optimize the rotation speed of the stepping motor for forming the planar lipid bilayer under control of a microcomputer. Next, we generate cell-sized liposomes using this automated system at the optimized rotation speed. This automatic system will contribute to high-throughput investigations of membrane protein activities and functions using the electrophysiological or the asymmetric liposome analyses.

元の言語English
ページ(範囲)57-63
ページ数7
ジャーナルSensors and Actuators, B: Chemical
292
DOI
出版物ステータスPublished - 2019 8 1

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Liposomes
lipids
stepping motors
cells
Lipids
Stepping motors
microcomputers
manipulators
time measurement
Lipid bilayers
membranes
proteins
Cell membranes
Microfluidics
Microcomputers
Membrane Proteins
Throughput
Proteins
Membranes
Chemical analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

これを引用

Automatic generation system of cell-sized liposomes. / Gotanda, Masahide; Kamiya, Koki; Osaki, Toshihisa; Miki, Norihisa; Takeuchi, Shoji.

:: Sensors and Actuators, B: Chemical, 巻 292, 01.08.2019, p. 57-63.

研究成果: Article

Gotanda, Masahide ; Kamiya, Koki ; Osaki, Toshihisa ; Miki, Norihisa ; Takeuchi, Shoji. / Automatic generation system of cell-sized liposomes. :: Sensors and Actuators, B: Chemical. 2019 ; 巻 292. pp. 57-63.
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