Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis

Yoshihiro Yasui, Takeshi Urano, Aie Kawajiri, Koh Ichi Nagata, Masaaki Tatsuka, Hideyuki Saya, Koichi Furukawa, Toshitada Takahashi, Ichiro Izawa, Masaki Inagaki

研究成果: Article査読

177 被引用数 (Scopus)

抄録

Mitotic kinases regulate cell division and its checkpoints, errors of which can lead to aneuploidy or genetic instability. One of these is Aurora-B, a key kinase that is required for chromosome alignment at the metaphase plate and for cytokinesis in mammalian cells. We report here that human Aurora-B is phosphorylated at Thr-232 through interaction with the inner centromere protein (INCENP) in vivo. The phosphorylation of Thr-232 occurs by means of an autophosphorylation mechanism, which is indispensable for the Aurora-B kinase activity. The activation of Aurora-B spatio-temporally correlated with the site-specific phosphorylation of its physiological substrates, histone H3 and vimentin. Overexpression of the TA mutant of Aurora-B, in which Thr-232 was changed into alanine, frequently induced multinuclearity in cells. These results indicate that the phosphorylation of Thr-232 is an essential regulatory mechanism for Aurora-B activation.

本文言語English
ページ(範囲)12997-13003
ページ数7
ジャーナルJournal of Biological Chemistry
279
13
DOI
出版ステータスPublished - 2004 3 26
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

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