Axenic cultivation of Entamoeba dispar in newly designed yeast extract-iron-gluconic acid-dihyrdoxyacetone-serum medium

Seiki Kobayashi, Eiko Imai, Ali Haghighi, Shaden A. Khalifa, Hiroshi Tachibana, Tsutomu Takeuchi

研究成果: Article査読

31 被引用数 (Scopus)

抄録

Yeast extract-iron-gluconic acid-dihyrdoxyacetone-serum medium that allows axenic cultivation of Entamoeba dispar was designed based on casein-free yeast extract-iron-serum (YI-S) medium, and the usefulness of the medium was assessed. The main differences from YI-S medium are replacement of glucose by gluconic acid, addition of dihydroxyacetone and D-galacturonic acid monohydrate, and sterilization by filtration. This medium promoted the axenic growth of 5 strains of E. dispar (2 strains of nonhuman primate isolates and 3 strains of human isolates). In addition, to clarify the biological basis for the growth of E. dispar in this medium, analyses of relevant enzymes on the glycolytic pathway of the amoebae as well as of the protozoans that are the best culture supplement for amoebae are being performed.

本文言語English
ページ(範囲)1-4
ページ数4
ジャーナルJournal of Parasitology
91
1
DOI
出版ステータスPublished - 2005 2
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 生態、進化、行動および分類学

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