Backbone resonance assignments for G protein α(i3) subunit in the GDP-bound state

Yoko Mase, Mariko Yokogawa, Masanori Osawa, Ichio Shimada

研究成果: Article

4 引用 (Scopus)

抄録

Guanine-nucleotide binding proteins (G proteins) serve as molecular switches in signaling pathways, by coupling the activation of G protein-coupled receptors (GPCRs) at the cell surface to intracellular responses. In the resting state, G protein forms a heterotrimer, consisting of the G protein α subunit with GDP (Gα·GDP) and the G protein βγ subunit (Gβγ). Ligand binding to GPCRs promotes the GDP-GTP exchange on Gα, leading to the dissociation of the GTP-bound form of Gα (Gα·GTP) and Gβγ. Then, Gα·GTP and Gβγ bind to their downstream effector enzymes or ion channels and regulate their activities, leading to a variety of cellular responses. Finally, Gα hydrolyzes the bound GTP to GDP and returns to the resting state by re-associating with Gβγ. The G proteins are classified with four major families based on the amino acid sequences of Gα: i/o, s, q/11, and 12/13. Here, we established the backbone resonance assignments of human Gαi3, a member of the i/o family with a molecular weight of 41 K, in complex with GDP. The chemical shifts were compared with those of Gα(i3) in complex with a GTP-analogue, GTPγS, which we recently reported, indicating that the residues with significant chemical shift differences are mostly consistent with the regions with the structural differences between the GDP- and GTPγS-bound states, as indicated in the crystal structures. The assignments of Gα(i3)·GDP would be useful for the analyses of the dynamics of Gα(i3) and its interactions with various target molecules.

元の言語English
ページ(範囲)237-241
ページ数5
ジャーナルBiomolecular NMR Assignments
8
発行部数2
DOI
出版物ステータスPublished - 2014 10 1
外部発表Yes

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Protein Subunits
GTP-Binding Proteins
Guanosine Triphosphate
gamma-Glutamyltransferase
Chemical shift
G-Protein-Coupled Receptors
Guanine Nucleotides
Ion Channels
Amino Acid Sequence
Ion exchange
Carrier Proteins
Crystal structure
Molecular Weight
Chemical activation
Molecular weight
Switches
Ligands
Amino Acids
Molecules
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

これを引用

Backbone resonance assignments for G protein α(i3) subunit in the GDP-bound state. / Mase, Yoko; Yokogawa, Mariko; Osawa, Masanori; Shimada, Ichio.

:: Biomolecular NMR Assignments, 巻 8, 番号 2, 01.10.2014, p. 237-241.

研究成果: Article

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