Bacteria manipulation using dielectrophoresis for efficient screening

T. Kano, T. Inaba, N. Miki

研究成果: Conference contribution

抄録

In this paper we demonstrate a microfluidic device that immobilizes microbes into microporous carrier using positive dielectrophoresis (DEP). This device can characterize bacteria, while precisely controlling the reaction conditions and the number of bacteria involved in the biochemical reaction, and can effectively screen them to efficiently produce useful chemicals by fermentation, such as organic acids, antibiotic drugs, and foods. We chose microbes belonging to Corynebacterium group as samples. First, as preparing for using the microfluidic device, we experimentally deduced effective DEP frequency of 10 MHz, when live C. glutamicum was preferably driven to the areas with stronger electric fields. Second, we characterized the microfluidic device and found that the device could immobilize microbes into micro pores and evaluate the production capacity of a single bacrerium. As the demonstration, we used the developed device to characterize microbes under various environments and evaluate biological activity.

本文言語English
ホスト出版物のタイトル2012 IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2012
ページ3247-3252
ページ数6
DOI
出版ステータスPublished - 2012 12 1
イベント25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012 - Vilamoura, Algarve, Portugal
継続期間: 2012 10 72012 10 12

出版物シリーズ

名前IEEE International Conference on Intelligent Robots and Systems
ISSN(印刷版)2153-0858
ISSN(電子版)2153-0866

Other

Other25th IEEE/RSJ International Conference on Robotics and Intelligent Systems, IROS 2012
CountryPortugal
CityVilamoura, Algarve
Period12/10/712/10/12

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Vision and Pattern Recognition
  • Computer Science Applications

フィンガープリント 「Bacteria manipulation using dielectrophoresis for efficient screening」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル