Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Hideyuki Nishizawa, Mami Iida, Yoshihiro Abe

研究成果: Article査読

1 被引用数 (Scopus)

抄録

When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.

本文言語English
ページ(範囲)451-454
ページ数4
ジャーナルanalytical sciences
1
5
DOI
出版ステータスPublished - 1985
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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