Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis

Hideyuki Nishizawa, Mami Iida, Yoshihiro Abe

研究成果: Article

1 引用 (Scopus)

抄録

When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.

元の言語English
ページ(範囲)451-454
ページ数4
ジャーナルAnalytical Sciences
1
発行部数5
DOI
出版物ステータスPublished - 1985

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Electrophoresis
Acids
Acetic Acid
Coloring Agents
Gels
Proteins
polyacrylamide gels

ASJC Scopus subject areas

  • Analytical Chemistry

これを引用

Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis. / Nishizawa, Hideyuki; Iida, Mami; Abe, Yoshihiro.

:: Analytical Sciences, 巻 1, 番号 5, 1985, p. 451-454.

研究成果: Article

Nishizawa, Hideyuki ; Iida, Mami ; Abe, Yoshihiro. / Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis. :: Analytical Sciences. 1985 ; 巻 1, 番号 5. pp. 451-454.
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