抄録
When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 451-454 |
| ページ数 | 4 |
| ジャーナル | Analytical Sciences |
| 巻 | 1 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | Published - 1985 |
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ASJC Scopus subject areas
- Analytical Chemistry
これを引用
Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis. / Nishizawa, Hideyuki; Iida, Mami; Abe, Yoshihiro.
：: Analytical Sciences, 巻 1, 番号 5, 1985, p. 451-454.研究成果: Article
}
TY - JOUR
T1 - Band Sharpening by Acid in Polyacrylamide Gel Electrophoresis
AU - Nishizawa, Hideyuki
AU - Iida, Mami
AU - Abe, Yoshihiro
PY - 1985
Y1 - 1985
N2 - When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.
AB - When we used an acidic solution of BPB as a marker for tracking, we observed that the band of the dye sharpened to become a very thin line. We supposed that the sample proteins in the gel should be similarly concentrated by the action of acid, and that the resolution should be improved. Among the acids we assessed, most of which sharpened the BPB band, 2 M acetic acid showed the proper band sharpening action and brought about the improvement in resolution.
KW - band sharpening
KW - Bromophenol Blue (BPB)
KW - Polyacrylamide gel electrophoresis (PAGE)
UR - http://www.scopus.com/inward/record.url?scp=85004464964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004464964&partnerID=8YFLogxK
U2 - 10.2116/analsci.1.451
DO - 10.2116/analsci.1.451
M3 - Article
AN - SCOPUS:85004464964
VL - 1
SP - 451
EP - 454
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 5
ER -