Basement membrane-like matrix sponge for the three-dimensional proliferation culture of differentiated retinal horizontal interneurons

Itsuki Ajioka, Shizuko Ichinose, Kazunori Nakajima, Hidehiro Mizusawa

研究成果: Article査読

8 被引用数 (Scopus)

抄録

As the neurons in the central nervous system (CNS) form a neuronal network in a three-dimensional (3D) manner, a 3D proliferation culture system for differentiated neurons has been desired. Although differentiated neurons were previously thought to never proliferate, differentiated horizontal interneurons of Rb-/-; p107+. /-; p130-/- (p107-single) retina clonally proliferated without dedifferentiation in vivo [1]. In the present study, we developed a basement membrane-like matrix sponge (BM-sponge) for the 3D proliferation culture of differentiated horizontal interneurons. p107-single horizontal interneurons, but not other types of retinal neurons, proliferated in the BM-sponge in a 3D manner. These interneurons expressed presynaptic marker and developed synaptic vesicles. These data demonstrated that p107-single horizontal interneurons cultured in the BM-sponge proliferate while maintaining their differentiated features. We described here the 3D proliferation culture system for differentiated neurons.

本文言語English
ページ(範囲)5765-5772
ページ数8
ジャーナルBiomaterials
32
25
DOI
出版ステータスPublished - 2011 9

ASJC Scopus subject areas

  • バイオエンジニアリング
  • セラミックおよび複合材料
  • 生物理学
  • 生体材料
  • 材料力学

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