TY - JOUR
T1 - Bcl-2-independent induction of apoptosis by neuropeptide receptor antagonist in human small cell lung carcinoma cells
AU - Matsumoto, Y.
AU - Kawatani, M.
AU - Simizu, S.
AU - Tanaka, T.
AU - Takada, M.
AU - Imoto, M.
PY - 2000/11/9
Y1 - 2000/11/9
N2 - The broad-spectrum antagonist of neuropeptide receptor, [D-Arg1, D-Phe5, D-Trp7,9, Leu17]substance P, induced apoptosis selectively in human small cell lung carcinoma (SCLC) cells, which express gastrin-releasing peptide receptor, but not in other types of tumor cells as well as normal cells. The addition of gastrin-releasing peptide or bombesin and the inhibitor of caspase-3 suppressed [D-Arg1, D-Phe5, D-Trp7,9, Leu11]substance P-induced apoptosis. Moreover, [D-Arg1, D-Phe5, D-Trp7,9, Leu11]substance P-induced apoptosis was not suppressed by Bcl-2 over-expression. Thus, blockage of gastrin-releasing peptide receptor-mediated signaling may provide a novel therapeutic option in SCLC which has become resistant to conventional chemotherapeutic agents.
AB - The broad-spectrum antagonist of neuropeptide receptor, [D-Arg1, D-Phe5, D-Trp7,9, Leu17]substance P, induced apoptosis selectively in human small cell lung carcinoma (SCLC) cells, which express gastrin-releasing peptide receptor, but not in other types of tumor cells as well as normal cells. The addition of gastrin-releasing peptide or bombesin and the inhibitor of caspase-3 suppressed [D-Arg1, D-Phe5, D-Trp7,9, Leu11]substance P-induced apoptosis. Moreover, [D-Arg1, D-Phe5, D-Trp7,9, Leu11]substance P-induced apoptosis was not suppressed by Bcl-2 over-expression. Thus, blockage of gastrin-releasing peptide receptor-mediated signaling may provide a novel therapeutic option in SCLC which has become resistant to conventional chemotherapeutic agents.
KW - Apoptosis
KW - Bcl-2
KW - Gastrin-releasing peptide
KW - Small cell lung carcinoma
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UR - http://www.scopus.com/inward/citedby.url?scp=0033785521&partnerID=8YFLogxK
M3 - Article
C2 - 11062732
AN - SCOPUS:0033785521
VL - 20
SP - 3123
EP - 3129
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 5
ER -
