Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus

Tsutomu Sasaki, Kazuo Kitagawa, Yoshiki Yagita, Shiro Sugiura, Emi Omura-Matsuoka, Shigeru Tanaka, Kohji Matsushita, Hideyuki Okano, Yoshihide Tsujitnoto, Masatsugu Hori

研究成果: Article査読

66 被引用数 (Scopus)

抄録

Neuronal progenitors in the adult hippocampus continually proliferate and differentiate to the neuronal lineage, and ischemic insult promotes hippocampal neurogenesis. However, newborn neurons show a progressive reduction in numbers during the initial few weeks, therefore, enhanced survival of newborn neurons seems to be essential for therapeutic strategy. Bcl-2 is a crucial regulator of programmed cell death in CNS development and in apoptotic and necrotic cell death. Therefore, we tested whether Bcl-2 overexpression enhances survival of newborn neurons in the adult mouse hippocampus under normal and ischemic conditions. Many newborn neurons in the hippocampal dentate gyrus undergo apoptosis. Human Bcl-2 expression in NSE-bcl-2 transgenic mice began at the immature neuronal stage and remained constant in surviving mature neurons. Bcl-2 significantly increased survival of newborn neurons under both conditions, but particularly after ischemia, with decreased cell death of newborn neurons in NSE-bcl-2 transgenic mice. We also clarified the effect by Bcl-2 overexpression of enhanced survival of newborn neurons in primary hippocampal cultures with BrdU labeling. These findings suggest that Bcl-2 plays a crucial role in adult hippocampal neurogenesis under normal and ischemic conditions.

本文言語English
ページ(範囲)1187-1196
ページ数10
ジャーナルJournal of neuroscience research
84
6
DOI
出版ステータスPublished - 2006 11月 1

ASJC Scopus subject areas

  • 細胞および分子神経科学

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