Bicistronic DNA display for in vitro selection of Fab fragments

Takeshi Sumida, Nobuhide Doi, Hiroshi Yanagawa

研究成果: Article

18 引用 (Scopus)

抜粋

In vitro display methods are superior tools for obtaining monoclonal antibodies. Although totally in vitro display methods, such as ribosome display and mRNA display, have the advantages of larger library sizes and quicker selection procedures compared with phage display, their applications have been limited to single-chain Fvs due to the requirement for linking of the mRNA and the nascent protein on the ribosome. Here we describe a different type of totally in vitro method, DNA display, that is applicable to heterodimeric Fab fragments: in vitro compartmentalization in water-in-oil emulsions allows the linking of an oligomeric protein and its encoding DNA with multiple ORFs. Since previously used emulsions impaired the synthesis of functional Fab fragments, we modified conditions for preparing emulsions, and identified conditions under which it was possible to enrich Fab fragments 106-fold per three rounds of affinity selection. Furthermore, we confirmed that genes encoding stable Fab fragments could be selected from a Fab fragment library with a randomized hydrophobic core in the constant region by applying heat treatment as a selection pressure. Since this method has all advantages of both phage display and totally in vitro display, it represents a new option for many applications using display methods.

元の言語English
記事番号gkp776
ページ(範囲)e147-e147
ジャーナルNucleic acids research
37
発行部数22
DOI
出版物ステータスPublished - 2009 9 29

ASJC Scopus subject areas

  • Genetics

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