Biocombinatorial selection of metal ion-chelating peptides

Teruhiko Matsubara, Yuko Hiura, Katsuhiro Kawashiro

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A phage-displayed library selection was performed to obtain metal ion-chelating peptides. A dodecamer (12-mer) random peptide library was displayed on the surface of filamentous bacterial phage and subjected to an affinity selection. Four rounds of the selection gave fourteen Zn2+-positive phage clones. Enzyme-linked immunosorbent assay showed that the selected clones specifically bound to Zn2+ and Ni2+, but not to Cu2+ and Fe3+. Deduced amino acid sequences of the clones had histidinerich consensus motifs. These chelating peptides should be applied to designing for metal ion-trapping biomaterials.

本文言語English
ページ(範囲)1324-1328
ページ数5
ジャーナルInternational Journal of Modern Physics B
17
8-9 I
DOI
出版ステータスPublished - 2003 4 10
外部発表はい

ASJC Scopus subject areas

  • 統計物理学および非線形物理学
  • 凝縮系物理学

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