TY - JOUR
T1 - Biocombinatorial selection of metal ion-chelating peptides
AU - Matsubara, Teruhiko
AU - Hiura, Yuko
AU - Kawashiro, Katsuhiro
PY - 2003/4/10
Y1 - 2003/4/10
N2 - A phage-displayed library selection was performed to obtain metal ion-chelating peptides. A dodecamer (12-mer) random peptide library was displayed on the surface of filamentous bacterial phage and subjected to an affinity selection. Four rounds of the selection gave fourteen Zn2+-positive phage clones. Enzyme-linked immunosorbent assay showed that the selected clones specifically bound to Zn2+ and Ni2+, but not to Cu2+ and Fe3+. Deduced amino acid sequences of the clones had histidinerich consensus motifs. These chelating peptides should be applied to designing for metal ion-trapping biomaterials.
AB - A phage-displayed library selection was performed to obtain metal ion-chelating peptides. A dodecamer (12-mer) random peptide library was displayed on the surface of filamentous bacterial phage and subjected to an affinity selection. Four rounds of the selection gave fourteen Zn2+-positive phage clones. Enzyme-linked immunosorbent assay showed that the selected clones specifically bound to Zn2+ and Ni2+, but not to Cu2+ and Fe3+. Deduced amino acid sequences of the clones had histidinerich consensus motifs. These chelating peptides should be applied to designing for metal ion-trapping biomaterials.
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U2 - 10.1142/s0217979203018946
DO - 10.1142/s0217979203018946
M3 - Article
AN - SCOPUS:0038778437
VL - 17
SP - 1324
EP - 1328
JO - International Journal of Modern Physics B
JF - International Journal of Modern Physics B
SN - 0217-9792
IS - 8-9 I
ER -