Biologically active human and mouse nerve growth factors secreted by the yeast Saccharomyces cerevisiae

Masafumi Nishizawa, Fumiko Ozawa, Takako Higashizaki, Keiko Hirai, Fumio Hishinuma

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Nerve growth factor (NGF) is a trophic agent that is essential for the development and survival of sympathetic and sensory nerves. A chemically-synthesized DNA fragment encoding human NGF (hNGF) and a cDNA encoding mouse NGF (mNGF) were engineered for expression in the yeast, Saccharomyces cerevisiae. Expression and secretion of hNGF and mNGF was attempted under the direction of the yeast PGK promoter and with various leader sequences. Among the leader sequences tested, that of the yeast α-factor successfully directed secretion of both hNGF and mNGF that were correctly processed. The content of the recombinant NGF (reNGF) in the culture supernatant was estimated to be 1 μg/ml. The yeast-produced reNGF was able to bind to NGF receptors in rat pheochromocytoma (PC12) cells as efficiently as the standard mNGF, and partially purified reNGF could induce neurite outgrowth of PC12 cells. Thus, we have demonstrated that biologically active human and mouse reNGF can be produced in yeast cells.

本文言語English
ページ(範囲)624-630
ページ数7
ジャーナルApplied Microbiology and Biotechnology
38
5
DOI
出版ステータスPublished - 1993 2月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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